380 Innovative Coencapsulation of Immunoactive and Anti-inflammatory Drugs for Treating Inflammatory Dermatoses

We aimed to quantify the biological effects of an innovative Pickering emulsion coencapsulating immunosuppressive drug (e.g.cyclosporinA) in biodegradable and biocompatible PLGA nanoparticles, with anti-inflammatory (e.g.calcitriol) oil phase, on T-cells keratinocytes vitro a murine model psoriasis establish proof concept efficacy vivo. were isolated from healthy volunteer’ blood or skin explants studied after CD3*CD28 activation during 6 days vitro, for IL-2 production (ELISA), T cell proliferation (CFSE), CD25 membrane expression (flow cytometry), while PMA+Ionomycin-mediated NFAT translocation was by immunofluorescence. Rhodamine-labeled nanoparticle penetration topical application evaluated human confocal microscopy. The imiquimod-induced van der Fits used Our results showed that emulsions significantly reduced T-cell NFAT-dependent proliferation. No cytotoxicity observed MTT whereas significant decrease their TNFα IL-8 (ELISA) occurred as compared controls. A increased rhodamine epidermal staining 24h-topical application. In vivo, reduction PASI score psoriasis-induced imiquimod detected Altogether, these demonstrated efficiently pro-inflammatory responses both cells and, through penetration, contribute lesions